Abstract The E3 ubiquitin ligases have been known to intrigue many researchers to date. due to their heterogenicity and substrate mediation for ubiquitin transfer to the protein. HECT (Homologous to the E6-AP Carboxyl Terminus) E3 ligases are spatially and temporally regulated for substrate specificity. E2 ubiquitin-conjugating enzyme interaction. https://www.rawafricaonline.com/